r/microscopy • • Jun 08 '23

πŸ¦ πŸ”¬πŸ¦ πŸ”¬πŸ¦  Microbe Identification Resources πŸ¦ πŸ”¬πŸ¦ πŸ”¬πŸ¦ 

149 Upvotes

πŸŽ‰Hello fellow microscopists!πŸŽ‰

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy • • Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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70 Upvotes

r/microscopy • • 13h ago

Photo/Video Share A small ciliate bites a Litonotus cygnus in the ass

166 Upvotes

Leitz Dialux II, Galaxy S24


r/microscopy • • 5h ago

Micro Art HAIR IN THE EYE

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24 Upvotes

HAIR IN THE EYE

Everyone knows what happens when a hair gets into the eye.

One tiny strand is enough to make us blink, rub, shed tears, and fumble about till we get it out. Eyes are made to see. Hair in the eye is a nuisance. Plain and simple.

Yet there is a creature that wears a whole forest of hair on its eyes.

The honeybee.

I came upon this odd sight when I put a honeybee's eye beneath a microscope lens.

At such close range, an eye no longer looks like an eye. It becomes a vast dome, roofed with thousands of tiny tiles, each laid neatly beside the next. Under slanting light, they shine blue and cold, like steel fresh from the forge.

And there, midst all those tiles, hair grows.

Real hair. With roots and tips. Some strands long, some short. Some stand tall, some lean sideways, and some reach out as though to feel what lies beyond.

How strange!

Nature took such pains to build an eye so finely made, then went and planted hair all over it.

Had it been a human eye, someone would have reached for the tweezers by now.

But the bee sees no need to mend a thing.

She still flies, still seeks out flowers, still finds her way home. Those hairs, which seem so much in the way, help keep dust from her eyes as she goes about her work.

So perhaps what looks like a hindrance may sometimes be part of what lets us see.

I sat looking at the photograph for quite a while.

On one side were the eye's tiny tiles, row upon row, neat and cold. On the other were the slender hairs, growing with no care for straight lines or tidy shapes. Light caught their tips, held there for a breath, then slipped away into the dark.

A world built with such care, yet filled with things that seem so out of place.

It made me think.

We humans like our eyes clear. We want the world before us sharp, clean, and set in order. When something gets in the way, we brush it aside. At times, even what we have yet to understand becomes something we would rather do without.

But the honeybee wears those hairs all her life.

No grumbling. No wish for a new pair of eyes.

I had meant to take a sharp photograph of an eye.

Instead, I found myself lost in the hairs growing upon it.

Perhaps, now and then, to see something worth seeing, we must stop trying to sweep everything out of sight.

Or perhaps there is no need to make so much of it.

The honeybee has hair growing in her eyes.

I have none.

Yet I still get so much wrong.

……


r/microscopy • • 12h ago

Photo/Video Share Apple flesh

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79 Upvotes

​

The microscopic view of fleshy part of the same apple I recently posted skin section of.

The cells evidently do not fill whole volume -- there are pores visible. Where cells do not touch other cells, their walls are domed, otherwise touching surfaces are almost flat.

Cells themselves are quite big -- 200 to 300 microns in size, but the only prominent parts are starch grains. It seems that the cells almost fully filled with vacuoles, and I couldn't discern cytoplasm and nucleus in them.

Images captured:

Objective: 10x.

Camera: 5Mp usb webcam with 0.5x reducer.

Focus stacking: Zerene Stacker.

Gif animation: ezgif.com free online tool.

Addition of grid and scale bar: Fiji/ImageJ.


r/microscopy • • 4h ago

Purchase Help Looking to buy a microscope

6 Upvotes

hi I'm new to the hobby, my 12-year-old has expressed an interest in microscopes and has outgrown the 100x

I'd like a microscope with binoc vision, decent overall features (lighting, magnification, ease of use) and the ability to record using either a PC/usb-c or else a mount for a phone/tablet. expecting to spend around $350. shipping to US (Philadelphia). size isn't much of a consideration, we've got plenty of space. mostly we're looking at samples we pull from nearby lakes, streams and swamps

is there an obvious brand/product for us?


r/microscopy • • 8h ago

ID Needed! Salt water mite? Any ideas on an ID?

13 Upvotes

Captured on an Olympus BH2, UPlanXApo 4x, Camera Nikon Fi2, salt water marsh pond sample.


r/microscopy • • 5h ago

ID Needed! Are these malassezia? Human sweat 1000x NSFW

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4 Upvotes

r/microscopy • • 9h ago

Photo/Video Share Home made Microscope showing cell wall.

3 Upvotes

I found a microscope lens which I made 10 vears ago when i was 14 yr old ..now I am a mbbs student and i can say its magnifying power is upto 40x..easily diagnose many diseases with this .it Can compete easily with microscope of thousands of rupee and i made it at home.

I wonder Indian education svstem never supports children creativity . No one never praise me for it..


r/microscopy • • 1d ago

Photo/Video Share Ramazzottius tardigrade

131 Upvotes

Just a lil gal


r/microscopy • • 20h ago

Photo/Video Share Vorticella doing some filter-feeding

19 Upvotes

Leitz Dialux II, 250x, Galaxy S24


r/microscopy • • 1d ago

ID Needed! Paramecium?

16 Upvotes

r/microscopy • • 11h ago

Photo/Video Share Biology and Microscopy of Planaria

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1 Upvotes

In this video we will take a look at the Biology and Microscopy of Planaria, which are atype of flatworm, often studied in biology due to their fascinating regenerative abilities. Using the Beaverlab M2 Digital Microscope.


r/microscopy • • 12h ago

ID Needed! Looking for ID help with a micro algae sample

1 Upvotes

https://reddit.com/link/1x8fahs/video/odngp8lf52wh1/player

Looking for a general ID for these microalgae from a biofilm in a freshwater pond sample that grew in a lab dish for several months. I am leaning toward Tetradesmus or Scenedesmus. Thanks for your shared expertise!

Motic BA310e 60X objective with an iLabcam Ultra adapter & an iPhone 15 Pro with 2x zoom.


r/microscopy • • 19h ago

Purchase Help MECHANIC ROBOT7060T-TG

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2 Upvotes

Has anyone used this specific microscope model for studying seeds or any type of macroremains? This microscope is supposedly oriented for technicians in those who work on repairing electronics, i know it's a stereo microscope like any other but I'm just skeptical and want to make sure it's suitable for that specific purpose, thank you.


r/microscopy • • 1d ago

Photo/Video Share Fennel (Foeniculum vulgare) Stomata on epidermis of the leaf

37 Upvotes

Using the red-blue computational extracted focus slice movies of the fennel cells and stomata to create the 3d point cloud and animate them, including some of the 2 second focus slice video to create the z-axis on the point cloud. Image contrast on living cell, computational blue red subtraction for the contrast Python OpenCV for the video, Python Open3D for the point cloud. Objective 60x amscope no oil, 40x olympus 10x olympus 0.5 reducer industrial camera (color) Video cut with OpenShot. Goal is to have super cheap imaging work-flow.


r/microscopy • • 1d ago

ID Needed! Nostoc sp. - Eterocyst visibile?

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16 Upvotes

Today at university, I had a microbiology lecture where we discussed cyanobacteria, specifically the structure of heterocysts and their role in nitrogen fixation.

While looking back at some photos I had taken with my microscope, I came across this spherical, filamentous thing. After a quick search, I discovered it's a cyanobacteria that belongs to the genus Nostoc.

Could anyone tell me if this larger cell (circled in the second pic) is a heterocyst?

Thanks in advance


r/microscopy • • 1d ago

General discussion Update the sim - Going opensource soon

15 Upvotes

r/microscopy • • 1d ago

Photo/Video Share Hairy Gastrotrich

35 Upvotes

Koristka Milano microscope (around 1920), magnified with phone camera


r/microscopy • • 1d ago

Photo/Video Share Fennel (Foeniculum vulgare) Stomata on epidermis of the leaf

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21 Upvotes

Using computational red-blue contrast 60x 40x 10x with 0.5 reducer and industrial camera real-time imaging of the living cells. Python OpenCV idle script direct capture USB port.


r/microscopy • • 1d ago

Photo/Video Share Tiny flagellate stuck inside a diatom shell

49 Upvotes

Leitz Dialux II, Galaxy S24


r/microscopy • • 1d ago

Photo/Video Share Colpoda ciliate

9 Upvotes

Koristka Milano microscope , magnified with phone camera


r/microscopy • • 2d ago

Techniques Some samples with and without filters

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64 Upvotes

Experimenting with some dark field and Rheinberg filters. On an off for comparison. Paramecium, stentor, and volvox.


r/microscopy • • 1d ago

Photo/Video Share Beckman Coulter Optima L-90K Ultracentrifuge

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1 Upvotes

r/microscopy • • 1d ago

Purchase Help Help adapting Sony A7s mirrorless camera to Bresser Researcher Trino Microscope

1 Upvotes

I am trying too figure out what to buy to attach my Sony A7s full frame mirrorless camera to my Bresser Researcher Trino Microscope. What i figured so far is that for best results i need the BRESSER SLR-camera-adapter 2x T2 23.2 mm and a T2 ring but it's way too expensive. Could i get away spending less with acceptable results? Like using a telescope 2x barlow , or not using any lens at all just some adapters.

Thank you, any insight is appreciated.